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cas-9 enzyme  (GeneSearch Inc)

 
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    GeneSearch Inc cas-9 enzyme
    Cas 9 Enzyme, supplied by GeneSearch Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cas-9 enzyme/product/GeneSearch Inc
    Average 90 stars, based on 1 article reviews
    cas-9 enzyme - by Bioz Stars, 2026-05
    90/100 stars

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    Effect of CRISPR-Cas9 mediated LINC00511 promoter deletion on HCC70 colony formation and paclitaxel sensitivity. ( a , b ) Genomic deletion of the LINC00511 promoter using CRISPR-Cas9 in the HCC70 TNBC model. qRT-PCR for LINC00511 expression in HCC70 WT and LINC00511-KO cell models. Data are presented as mean ± SD, n = 3. *** p < 0.0005. Clonogenic assay for HCC70 parental and LINC00511-KO cells, in the absence ( c , d ) or the presence ( e , f ) of indicated concentrations of Paclitaxel. Data are presented as mean ± SD, n = 4. *** p < 0.0005. ( g ) Cell cycle analysis for HCC70 parental and LINC00511-KO cells in the absence or presence of different concentrations of Paclitaxel.

    Journal: Non-Coding RNA

    Article Title: LncRNA-Based Classification of Triple Negative Breast Cancer Revealed Inherent Tumor Heterogeneity and Vulnerabilities

    doi: 10.3390/ncrna8040044

    Figure Lengend Snippet: Effect of CRISPR-Cas9 mediated LINC00511 promoter deletion on HCC70 colony formation and paclitaxel sensitivity. ( a , b ) Genomic deletion of the LINC00511 promoter using CRISPR-Cas9 in the HCC70 TNBC model. qRT-PCR for LINC00511 expression in HCC70 WT and LINC00511-KO cell models. Data are presented as mean ± SD, n = 3. *** p < 0.0005. Clonogenic assay for HCC70 parental and LINC00511-KO cells, in the absence ( c , d ) or the presence ( e , f ) of indicated concentrations of Paclitaxel. Data are presented as mean ± SD, n = 4. *** p < 0.0005. ( g ) Cell cycle analysis for HCC70 parental and LINC00511-KO cells in the absence or presence of different concentrations of Paclitaxel.

    Article Snippet: In brief, 1000 ng of each gRNA were mixed with 1.5 μL of 20 µM Cas-9 Enzyme (EnGen Spy Cas9 NLS (NEB# M0646M)) to form the RNP complex.

    Techniques: CRISPR, Quantitative RT-PCR, Expressing, Clonogenic Assay, Cell Cycle Assay